Ontology highlight
ABSTRACT:
SUBMITTER: Kamalampeta R
PROVIDER: S-EPMC3443816 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Kamalampeta Rajashekhar R Kothe Ute U
Scientific reports 20120917
Cbf5 is a pseudouridine synthase that usually acts in a guide RNA-dependent manner as part of H/ACA small ribonucleoproteins; however archaeal Cbf5 can also act independently of guide RNA in modifying uridine 55 in tRNA. This guide-independent activity of Cbf5 is enhanced by proteins Nop10 and Gar1 which are also found in H/ACA small ribonucleoproteins. Here, we analyzed the specific contribution of Nop10 and Gar1 for Cbf5-catalyzed pseudouridylation of tRNA. Interestingly, both Nop10 and Gar1 n ...[more]