Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Nanopore-Based Sensors for Detecting Toxicity of a Carbon Nanotube to Proteins.

ABSTRACT: A carbon nanotube (CNT) can be toxic to a living cell by binding to proteins and then impairing their functionalities; however, an efficient screening method that examines binding capability of a CNT to protein molecules in vitro is still unavailable. Here, we show that a nanopore-based sensor can be used to investigate CNT-protein interactions. With proof-of-principle molecular dynamics simulations, we have measured ionic currents in a nanopore when threading a CNT-protein complex through the pore, and demonstrated that CNT's binding capability, and thus potential nanotoxicity, can be inferred from current signals. We have then further investigated mechanics and energetics of CNT-protein interactions with the nanopore sensor. These findings indicate that solid-state nanopores have the potential to be ultra-sensitive and high-throughput sensors for nanotoxicity.

SUBMITTER: Luan B

PROVIDER: S-EPMC3445412 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Publications

Nanopore-Based Sensors for Detecting Toxicity of a Carbon Nanotube to Proteins.

Luan Binquan B Zhou Ruhong R

The journal of physical chemistry letters 20120801 3

A carbon nanotube (CNT) can be toxic to a living cell by binding to proteins and then impairing their functionalities; however, an efficient screening method that examines binding capability of a CNT to protein molecules in vitro is still unavailable. Here, we show that a nanopore-based sensor can be used to investigate CNT-protein interactions. With proof-of-principle molecular dynamics simulations, we have measured ionic currents in a nanopore when threading a CNT-protein complex through the p ...[more]

PMID: 23002420

Similar Datasets

Functionalized carbon nanotube toxicity in Arabidopsis leaves

Project description:Polyethilenimine (PEI) functionalized single walled carbon nanotubes (SWNTs) and carbon-based nanomaterials enable delivery of DNA and RNA in plants. Given the broad-scale use of PEI-functionalized nanomaterials in plants, we sought to investigate the reaction of plant tissues to treatment with PEI-SWNTs and pristine SWNTs. To this end, we infiltrated Arabidopsis thaliana leaves with pristine single walled carbon nanotubes used in RNA silencing applications (SWNTs) and polyethyleneimine-functionalized SWNTs used for plasmid DNA delivery (PEI-SWNTs). We used Arabidopsis as it is a well characterized model plant, for which genomic and detailed gene function information is readily available. To minimize the effects caused by the introduction of exogenous nucleic acids, in SWNT preparations we used single stranded RNA targeting Green Fluorescent Protein (GFP) with no target sequence in the Arabidopsis genome, and a plasmid that expresses GFP in PEI-SWNT preparations. For our experiments herein, we used ~25-50 fold higher concentrations of SWNTs and PEI-SWNTs compared to standard concentrations used in biomolecule delivery assays. Water-infiltrated plant leaves served as a negative control to distinguish between the SWNT-specific response and the response to the infiltration process itself. We performed RNA sequencing (RNA-seq) with RNA extracted from leaves two days after infiltration to identify changes in the leaf transcriptomic profile in response to the three treatments, compared to non-infiltrated leaves.

2021-12-29 | GSE172278 | GEO

Tuning Selectivity of Fluorescent Carbon Nanotube-Based Neurotransmitter Sensors.

Project description:Detection of neurotransmitters is an analytical challenge and essential to understand neuronal networks in the brain and associated diseases. However, most methods do not provide sufficient spatial, temporal, or chemical resolution. Near-infrared (NIR) fluorescent single-walled carbon nanotubes (SWCNTs) have been used as building blocks for sensors/probes that detect catecholamine neurotransmitters, including dopamine. This approach provides a high spatial and temporal resolution, but it is not understood if these sensors are able to distinguish dopamine from similar catecholamine neurotransmitters, such as epinephrine or norepinephrine. In this work, the organic phase (DNA sequence) around SWCNTs was varied to create sensors with different selectivity and sensitivity for catecholamine neurotransmitters. Most DNA-functionalized SWCNTs responded to catecholamine neurotransmitters, but both dissociation constants (Kd) and limits of detection were highly dependent on functionalization (sequence). Kd values span a range of 2.3 nM (SWCNT-(GC)15 + norepinephrine) to 9.4 μM (SWCNT-(AT)15 + dopamine) and limits of detection are mostly in the single-digit nM regime. Additionally, sensors of different SWCNT chirality show different fluorescence increases. Moreover, certain sensors (e.g., SWCNT-(GT)10) distinguish between different catecholamines, such as dopamine and norepinephrine at low concentrations (50 nM). These results show that SWCNTs functionalized with certain DNA sequences are able to discriminate between catecholamine neurotransmitters or to detect them in the presence of interfering substances of similar structure. Such sensors will be useful to measure and study neurotransmitter signaling in complex biological settings.

| S-EPMC5539566 | biostudies-other

Thin and Flexible Carbon Nanotube-Based Pressure Sensors with Ultrawide Sensing Range.

Project description:A scalable electrophoretic deposition (EPD) approach is used to create novel thin, flexible, and lightweight carbon nanotube-based textile pressure sensors. The pressure sensors can be produced using an extensive variety of natural and synthetic fibers. These piezoresistive sensors are sensitive to pressures ranging from the tactile range (<10 kPa), the body weight range (?500 kPa), and very high pressures (?40 MPa). The EPD technique enables the creation of a uniform carbon nanotube-based nanocomposite coating, in the range of 250-750 nm thick, of polyethyleneimine (PEI) functionalized carbon nanotubes on nonconductive fibers. In this work, nonwoven aramid fibers are coated by EPD onto a backing electrode followed by film formation onto the fibers creating a conductive network. The electrically conductive nanocomposite coating is firmly bonded to the fiber surface and shows piezoresistive electrical/mechanical coupling. The pressure sensor displays a large in-plane change in electrical conductivity with applied out-of-plane pressure. In-plane conductivity change results from fiber/fiber contact as well as the formation of a sponge-like piezoresistive nanocomposite "interphase" between the fibers. The resilience of the nanocomposite interphase enables sensing of high pressures without permanent changes to the sensor response, showing high repeatability.

| S-EPMC6286676 | biostudies-literature

Super-stretchable, transparent carbon nanotube-based capacitive strain sensors for human motion detection.

Project description:Realization of advanced bio-interactive electronic devices requires mechanically compliant sensors with the ability to detect extremely large strain. Here, we design a new multifunctional carbon nanotube (CNT) based capacitive strain sensors which can detect strains up to 300% with excellent durability even after thousands of cycles. The CNT-based strain gauge devices exhibit deterministic and linear capacitive response throughout the whole strain range with a gauge factor very close to the predicted value (strictly 1), representing the highest sensitivity value. The strain tests reveal the presented strain gauge with excellent dynamic sensing ability without overshoot or relaxation, and ultrafast response at sub-second scale. Coupling these superior sensing capabilities to the high transparency, physical robustness and flexibility, we believe the designed stretchable multifunctional CNT-based strain gauge may have various potential applications in human friendly and wearable smart electronics, subsequently demonstrated by our prototypical data glove and respiration monitor.

| S-EPMC6505716 | biostudies-literature

Functionalized carbon nanotube toxicity in Arabidopsis leaves

Project description:Functionalized carbon nanotube toxicity in Arabidopsis leaves

| PRJNA722743 | ENA

In vitro gastrointestinal gas monitoring with carbon nanotube sensors.

Project description:In vitro simulators of the human gastrointestinal (GI) tract are remarkable technological platforms for studying the impact of food on the gut microbiota, enabling continuous and real-time monitoring of key biomarkers. However, comprehensive real-time monitoring of gaseous biomarkers in these systems is required with a cost-effective approach, which has been challenging to perform experimentally to date. In this work, we demonstrate the integration and in-line use of carbon nanotube (CNT)-based chemiresitive gas sensors coated with a thin polydimethylsiloxane (PDMS) membrane for the continuous monitoring of gases within the Simulator of the Human Microbial Ecosystem (SHIME). The findings demonstrate the ability of the gas sensor to continuously monitor the different phases of gas production in this harsh, anaerobic, highly humid, and acidic environment for a long exposure time (16 h) without saturation. This establishes our sensor platform as an effective tool for real-time monitoring of gaseous biomarkers in in vitro systems like SHIME.

| S-EPMC10774382 | biostudies-literature

Hydrogen Sensors Based on Flexible Carbon Nanotube-Palladium Composite Sheets Integrated with Ripstop Fabric.

Project description:This work describes the design and fabrication of free-standing carbon nanotube-palladium (CNT-Pd) composite sheets for hydrogen gas sensing. The CNT-Pd composites were made by electroplating palladium onto a solvent-densified and oxygen plasma-treated CNT sheet. The latter was prepared using high purity CNTs drawn from a dense, vertically aligned array grown by chemical vapor deposition on silicon substrates. The CNT-Pd sheets were characterized by energy-dispersive spectroscopy, scanning electron microscopy, and X-ray diffraction. The amount of palladium in the composite was 16.5 wt % as measured via thermogravimetric analysis. Thin strips of the CNT-Pd sheets were assembled as chemiresistor sensors and tested for hydrogen gas detection. The sensors demonstrated a limit of detection of 0.1 mol % and displayed signal reversibility without the need for oxygen removal or heat treatment. A decrease in signal reversibility was observed after multiple exposure cycles; however, redensification with ethanol significantly restored the original reversibility. The sensor showed the Freundlich adsorption isotherm behavior when exposed to hydrogen. The material's potential application toward a wearable, flexible sensor was demonstrated by integrating the chemiresistor onto a fabric material using hot-press processing and testing the composite for hydrogen sensitivity.

| S-EPMC6964304 | biostudies-literature

Direct Ink Writing of Strained Carbon Nanotube-Based Sensors: Toward 4D Printable Soft Robotics.

Project description:Four-dimensional (4D) printing has attracted significant attention, because it enables structures to be reconfigured based on an external stimulus, realizing complex architectures that are useful for different applications. Nevertheless, most previously reported 4D-printed components have focused on actuators, which are just one part of a full soft robotic system. In this study, toward achieving fully 4D-printed systems, the design and direct ink writing of sensors with a straining mechanism that mimics the 4D effect are explored. Solution-processable carbon nanotubes (CNTs) were used as the sensing medium, and the effect of a heat-shrinkable shape-memory polymer-based substrate (i.e., potential 4D effect) on the electronic and structural properties of CNTs was assessed, followed by their application in various sensing devices. Herein, we reveal that substrate shrinking affords a more porous yet more conductive film owing to the compressive strain experienced by CNTs, leading to an increase in the carrier concentration. Furthermore, it improves the sensitivity of the devices without the need for chemical functionalization. Interestingly, the results show that, by engineering the potential 4D effect, the selectivity of the sensor can be tuned. Finally, the sensors were integrated into a fully 4D-printed flower structure, exhibiting their potential for different soft robotic applications.

| S-EPMC10976367 | biostudies-literature

Airflow-assisted dielectrophoresis to reduce the resistance mismatch in carbon nanotube-based temperature sensors.

Project description:The dielectrophoresis (DEP) method is used to fabricate sensor devices by assembling and aligning carbon nanotubes (CNTs) across electrode structures. The challenges of the method increase as the gap width between the electrodes increases. In this work, a novel DEP setup is proposed to reduce the resistance mismatch in manufacturing carbon nanotube-based sensors. The proposed setup utilizes hot airflow and thermal annealing to fabricate long-aligned multi-walled carbon nanotube (MWCNT) bridges across transparent electrodes with a gap width up to 75 μm. The best alignment results were obtained at airflow velocities between 1.5 m s-1 and 2.5 m s-1. The minimum variation in the resistance of the aligned bridges was 1.81% observed at a MWCNT concentration of 0.005 wt% and deposition time of 10 min. Long MWCNT bridges have many contact points that link MWCNTs to each other, making the contact resistance a robust indicator of the variation in the ambient temperature. The characteristics of the MWCNT bridges as a temperature sensor, including the response, sensitivity, and recovery, were investigated.

| S-EPMC9044456 | biostudies-literature

Multiwalled Carbon Nanotube-Based On-Body Patch Antenna for Detecting COVID-19-Affected Lungs.

Project description:A novel rectangular patch antenna based on multiwall carbon nanotubes has been designed and developed for assisting the initial detection of COVID-19-affected lungs. Due to their highly conductive nature, each nanotube echoes electromagnetic waves in a unique manner, influencing the increase in bandwidth. The proposed antenna operates at 6.63, 7.291, 7.29, and 7.22 GHz with a higher bandwidth classified as an ultrawide band and can be used on a human body phantom model because of its flexibility and decreased radiation qualities. Flame retardant 4 is chosen as a substrate with a uniform thickness of 1.62 mm due to its inexpensive cost and excellent electrical properties. The maximum specific absorption rate of the proposed antenna is obtained as 1.77 W/kg for 10 g of tissues. For testing purposes, a model including all the known features of COVID-19-affected lungs is developed. The designed antenna exhibits excellent performance in free space, normal lungs, and affected lung environments. It might be utilized as a first screening device for COVID-19 patients, especially in resource-constrained areas where traditional medical equipment such as X-ray and computerized tomography scans are scarce.

| S-EPMC9380818 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data