A seventh bacterial chlorophyll driving a large light-harvesting antenna.

ABSTRACT: The discovery of new chlorophyllous pigments would provide greater understanding of the mechanisms and evolution of photosynthesis. Bacteriochlorophyll f has never been observed in nature, although this name was proposed ~40 years ago based on structurally related compounds. We constructed a bacteriochlorophyll f-accumulating mutant of the green sulfur bacterium Chlorobaculum limnaeum, which originally produced bacteriochlorophyll e, by knocking out the bchU gene encoding C-20 methyltransferase based on natural transformation. This novel pigment self-aggregates in an in vivo light-harvesting antenna, the chlorosome, and exhibits a Q(y) peak of 705 nm, more blue-shifted than any other chlorosome reported so far; the peak overlaps the maximum (~700 nm) of the solar photon flux spectrum. Bacteriochlorophyll f chlorosomes can transfer light energy from core aggregated pigments to another bacteriochlorophyll in the chlorosomal envelope across an energy gap of ~100 nm, and is thus a promising material for development of new bioenergy applications.

SUBMITTER: Harada J 

PROVIDER: S-EPMC3445912 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature