Variational Bayes analysis of a photon-based hidden Markov model for single-molecule FRET trajectories.

ABSTRACT: Single-molecule fluorescence resonance energy transfer (smFRET) measurement is a powerful technique for investigating dynamics of biomolecules, for which various efforts have been made to overcome significant stochastic noise. Time stamp (TS) measurement has been employed experimentally to enrich information within the signals, while data analyses such as the hidden Markov model (HMM) have been successfully applied to recover the trajectories of molecular state transitions from time-binned photon counting signals or images. In this article, we introduce the HMM for TS-FRET signals, employing the variational Bayes (VB) inference to solve the model, and demonstrate the application of VB-HMM-TS-FRET to simulated TS-FRET data. The same analysis using VB-HMM is conducted for other models and th

SUBMITTER: Okamoto K 

PROVIDER: S-EPMC3446682 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature