Ontology highlight
ABSTRACT:
SUBMITTER: Pinato G
PROVIDER: S-EPMC3447186 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Pinato Giulietta G Cojoc Dan D Lien Linh Thuy LT Ansuini Alessio A Ban Jelena J D'Este Elisa E Torre Vincent V
Scientific reports 20120920
Guidance molecules, such as Sema3A or Netrin-1, induce growth cone (GC) repulsion or attraction. In order to determine the speed of action and efficiency of these guidance cues we developed an experimental procedure to deliver controlled amounts of these molecules. Lipid vesicles encapsulating 10-10(4) molecules of Sema3A or Netrin-1 were manipulated with high spatial and temporal resolution by optical tweezers and their photolysis triggered by laser pulses. Guidance molecules released from the ...[more]