Ontology highlight
ABSTRACT:
SUBMITTER: Chang S
PROVIDER: S-EPMC3447775 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
PloS one 20120920 9
We describe the design, construction and validation of a fluorescence sensor to measure activation by agonist of the m1 muscarinic cholinergic receptor, a prototypical class I G(q)-coupled receptor. The sensor uses an established general design in which Förster resonance energy transfer (FRET) from a circularly permuted CFP mutant to FlAsH, a selectively reactive fluorescein, is decreased 15-20% upon binding of a full agonist. Notably, the sensor displays essentially wild-type capacity to cataly ...[more]