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Isolation of DNA aptamers using micro free flow electrophoresis.

ABSTRACT: A micro free flow electrophoresis (?FFE) device was used to select DNA aptamers for human immunoglobulin E (IgE). The continuous nature of ?FFE allowed 1.8 × 10(14) sequences to be introduced over a period of 30 min, a 300-fold improvement in library size over capillary electrophoresis based selections (CE-SELEX). Four rounds of selection were performed within four days. Aptamers with low nM dissociation constants for IgE were identified after a single round of ?FFE selection.

SUBMITTER: Jing M

PROVIDER: S-EPMC3454500 | biostudies-literature | 2011 Nov

REPOSITORIES: biostudies-literature

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Isolation of DNA aptamers using micro free flow electrophoresis.

Jing Meng M Bowser Michael T MT

Lab on a chip 20110923 21

A micro free flow electrophoresis (μFFE) device was used to select DNA aptamers for human immunoglobulin E (IgE). The continuous nature of μFFE allowed 1.8 × 10(14) sequences to be introduced over a period of 30 min, a 300-fold improvement in library size over capillary electrophoresis based selections (CE-SELEX). Four rounds of selection were performed within four days. Aptamers with low nM dissociation constants for IgE were identified after a single round of μFFE selection. ...[more]

PMID: 21947169

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