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Discovery of a novel (+)-?-lactamase from Bradyrhizobium japonicum USDA 6 by rational genome mining.

ABSTRACT: A novel (+)-?-lactamase used for the resolution of racemic ?-lactam from Bradyrhizobium japonicum USDA 6 was found as a result of sequence-structure guided genome mining. It consists of 409 amino acids, only 49% of which are identical to the amino acid sequences of the known (+)-?-lactamase from Sulfolobus solfataricus. This is only the third (+)-?-lactamase gene to be reported.

SUBMITTER: Zhu S 

PROVIDER: S-EPMC3457094 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature

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Discovery of a novel (+)-γ-lactamase from Bradyrhizobium japonicum USDA 6 by rational genome mining.

Zhu Shaozhou S   Gong Cuiyu C   Song Dawei D   Gao Shuaihua S   Zheng Guojun G  

Applied and environmental microbiology 20120810 20

A novel (+)-γ-lactamase used for the resolution of racemic γ-lactam from Bradyrhizobium japonicum USDA 6 was found as a result of sequence-structure guided genome mining. It consists of 409 amino acids, only 49% of which are identical to the amino acid sequences of the known (+)-γ-lactamase from Sulfolobus solfataricus. This is only the third (+)-γ-lactamase gene to be reported. ...[more]

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