Inactivation of the ybdD gene in Lactococcus lactis increases the amounts of exported proteins.
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ABSTRACT:
SUBMITTER: Morello E
PROVIDER: S-EPMC3457487 | biostudies-literature | 2012 Oct
REPOSITORIES: biostudies-literature
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Inactivation of the ybdD gene in Lactococcus lactis increases the amounts of exported proteins.
Morello E E Nouaille S S Cortes-Perez N G NG Blugeon S S Medina L F C LF Azevedo V V Gratadoux J J JJ Bermúdez-Humarán L G LG Le Loir Y Y Langella P P
Applied and environmental microbiology 20120727 19
Random insertional mutagenesis performed on a Lactococcus lactis reporter strain led us to identify L. lactis ybdD as a protein-overproducing mutant. In different expression contexts, the ybdD mutant shows increased levels of exported proteins and therefore constitutes a new and attractive heterologous protein production host. This study also highlights the importance of unknown regulatory processes that play a role during protein secretion. ...[more]
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