Unknown

Dataset Information

0

R26R-GR: a Cre-activable dual fluorescent protein reporter mouse.

ABSTRACT: Green fluorescent protein (GFP) and its derivatives are the most widely used molecular reporters for live cell imagining. The development of organelle-specific fusion fluorescent proteins improves the labeling resolution to a higher level. Here we generate a R26 dual fluorescent protein reporter mouse, activated by Cre-mediated DNA recombination, labeling target cells with a chromatin-specific enhanced green fluorescence protein (EGFP) and a plasma membrane-anchored monomeric cherry fluorescent protein (mCherry). This dual labeling allows the visualization of mitotic events, cell shapes and intracellular vesicle behaviors. We expect this reporter mouse to have a wide application in developmental biology studies, transplantation experiments as well as cancer/stem cell lineage tracing.

SUBMITTER: Chen YT 

PROVIDER: S-EPMC3458011 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Green fluorescent protein (GFP) and its derivatives are the most widely used molecular reporters for live cell imagining. The development of organelle-specific fusion fluorescent proteins improves the labeling resolution to a higher level. Here we generate a R26 dual fluorescent protein reporter mouse, activated by Cre-mediated DNA recombination, labeling target cells with a chromatin-specific enhanced green fluorescence protein (EGFP) and a plasma membrane-anchored monomeric cherry fluorescent  ...[more]

Similar Datasets

Unknown Dual fluorescent reporter pig for Cre recombination: transgene placement at the ROSA26 locus.
| S-EPMC4099177 | biostudies-literature
Unknown Near-infrared fluorescent protein iRFP713 as a reporter protein for optogenetic vectors, a transgenic Cre-reporter rat, and other neuronal studies.
| S-EPMC5501963 | biostudies-literature
Unknown Secreted dual reporter assay with Gaussia luciferase and the red fluorescent protein mCherry.
| S-EPMC5722324 | biostudies-literature
Unknown The IRG mouse: a two-color fluorescent reporter for assessing Cre-mediated recombination and imaging complex cellular relationships in situ.
| S-EPMC2928670 | biostudies-literature
Unknown A transgenic mouse class-III beta tubulin reporter using yellow fluorescent protein.
| S-EPMC2814058 | biostudies-literature
Unknown Modelling genetic mosaicism of neurodevelopmental disorders in vivo by a Cre-amplifying fluorescent reporter.
| S-EPMC7713426 | biostudies-literature
Unknown A transgenic Prox1-Cre-tdTomato reporter mouse for lymphatic vessel research.
| S-EPMC4388455 | biostudies-literature
Unknown Neuroanatomical characterization of a growth hormone secretagogue receptor-green fluorescent protein reporter mouse.
| S-EPMC4142102 | biostudies-literature
Unknown A single reporter mouse line for Vika, Flp, Dre, and Cre-recombination.
| S-EPMC6160450 | biostudies-literature
Unknown G2R Cre reporter transgenic zebrafish.
| S-EPMC2665268 | biostudies-literature