Ontology highlight
ABSTRACT:
SUBMITTER: Stengel F
PROVIDER: S-EPMC3458707 | biostudies-literature | 2012 May
REPOSITORIES: biostudies-literature
Stengel Florian F Baldwin Andrew J AJ Bush Matthew F MF Hilton Gillian R GR Lioe Hadi H Basha Eman E Jaya Nomalie N Vierling Elizabeth E Benesch Justin L P JL
Chemistry & biology 20120501 5
Small heat-shock proteins (sHSPs) are molecular chaperones that prevent irreversible aggregation through binding nonnative target proteins. Due to their heterogeneity, these sHSP:target complexes remain poorly understood. We present a nanoelectrospray mass spectrometry analysis algorithm for estimating the distribution of stoichiometries comprising a polydisperse ensemble of oligomers. We thus elucidate the organization of complexes formed between sHSPs and different target proteins. We find tha ...[more]