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A simple and efficient dual optical signaling chemodosimeter for toxic Hg(II).

ABSTRACT: 10-Methylthioacridone, designated as acrithion, has been employed as an easily accessible chemodosimeter for the optical targeting of toxic Hg(2+) in buffered aqueous DMSO. The Hg(2+)-mediated desulfurization of the probe is translated into selective dual signaling of Hg(2+) by means of color bleaching and fluorescence amplification while several other metal ions, including potentially competing Ag(+), Cu(2+) and Pb(2+), afford no significant interferences even in excess concentrations.

SUBMITTER: Mashraqui SH

PROVIDER: S-EPMC3458760 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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A simple and efficient dual optical signaling chemodosimeter for toxic Hg(II).

Mashraqui Sabir H SH Tripathi Sapna A SA Ghorpade Sushil S SS Britto Smita R SR

Beilstein journal of organic chemistry 20120822

10-Methylthioacridone, designated as acrithion, has been employed as an easily accessible chemodosimeter for the optical targeting of toxic Hg(2+) in buffered aqueous DMSO. The Hg(2+)-mediated desulfurization of the probe is translated into selective dual signaling of Hg(2+) by means of color bleaching and fluorescence amplification while several other metal ions, including potentially competing Ag(+), Cu(2+) and Pb(2+), afford no significant interferences even in excess concentrations. ...[more]

PMID: 23019470

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Project description:Optical spectroscopy and reverse-phase HPLC were used to investigate the binding of Hg(II) to plant metal-binding peptides (phytochelatins) with the structure (gammaGlu-Cys)2Gly, (gammaGlu-Cys)3Gly and (gammaGlu-Cys)4Gly. Glutathione-mediated transfer of Hg(II) into phytochelatins and the transfer of the metal ion from one phytochelatin to another was also studied using reverse-phase HPLC. The saturation of Hg(II)-induced bands in the UV/visible and CD spectra of (gammaGlu-Cys)2Gly suggested the formation of a single Hg(II)-binding species of this peptide with a stoichiometry of one metal ion per peptide molecule. The separation of apo-(gammaGlu-Cys)2Gly from its Hg(II) derivative on a C18 reverse-phase column also indicated the same metal-binding stoichiometry. The UV/visible spectra of both (gammaGlu-Cys)3Gly and (gammaGlu-Cys)4Gly at pH 7.4 showed distinct shoulders in the ligand-to-metal charge-transfer region at 280-290 mm. Two distinct Hg(II)-binding species, occurring at metal-binding stoichiometries of around 1.25 and 2.0 Hg(II) ions per peptide molecule, were observed for (gammaGlu-Cys)3Gly. These species exhibited specific spectral features in the charge-transfer region and were separable by HPLC. Similarly, two main Hg(II)-binding species of (gammaGlu-Cys)4Gly were observed by UV/visible and CD spectroscopy at metal-binding stoichiometries of around 1.25 and 2.5 respectively. Only a single peak of Hg(II)-(gammaGlu-Cys)4Gly complexes was resolved under the conditions used for HPLC. The overall Hg(II)-binding stoichiometries of phytochelatins were similar at pH 2.0 and at pH 7.4, indicating that pH did not influence the final Hg(II)-binding capacity of these peptides. The reverse-phase HPLC assays indicated a rapid transfer of Hg(II) from glutathione to phytochelatins. These assays also demonstrated a facile transfer of the metal ion from shorter- to longer-chain phytochelatins. The strength of Hg(II) binding to glutathione and phytochelatins followed the order: gammaGlu-Cys-Gly<(gammaGlu-Cys)2Gly<(gammaGlu-Cy s)3Gly<(gamma Glu-Cys)4Gly.

Dataset Information

A simple and efficient dual optical signaling chemodosimeter for toxic Hg(II).

Publications

A simple and efficient dual optical signaling chemodosimeter for toxic Hg(II).

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