Tear lacritin levels by age, sex, and time of day in healthy adults.

ABSTRACT:

Purpose

Several small proteomic studies suggest that the prosecretory tear protein lacritin may be selectively downregulated in dry eye syndrome and in blepharitis, yet little information is available about normal baseline levels. This study assessed lacritin levels in tears from healthy individuals and addressed whether they differ according to sex, age, or time of day.

Methods

Rabbit antibodies against lacritin N-terminal peptide EDASSDSTGADPAQEAGTS (Pep Lac N-Term) were generated and characterized against human recombinant lacritin and N-65 truncation mutant. Basal tears were collected from 66 healthy individuals ranging in age from 18 to 52 years, and at four times during one 24-hour period from 34 other individuals. Lacritin levels were then analyzed by ELISA and Wester

SUBMITTER: Seifert K 

PROVIDER: S-EPMC3460390 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature