Ontology highlight
ABSTRACT:
SUBMITTER: Chen CC
PROVIDER: S-EPMC3460417 | biostudies-literature | 2012 Sep
REPOSITORIES: biostudies-literature
Chen Chun-Chang CC Wang Keh-Yang KY Shen Che-Kun James CK
The Journal of biological chemistry 20120816 40
For cytosine (C) demethylation of vertebrate DNA, it is known that the TET proteins could convert 5-methyl C (5-mC) to 5-hydroxymethyl C (5-hmC). However, DNA dehydroxymethylase(s), or enzymes able to directly convert 5-hmC to C, have been elusive. We present in vitro evidence that the mammalian de novo DNA methyltransferases DNMT3A and DNMT3B, but not the maintenance enzyme DNMT1, are also redox-dependent DNA dehydroxymethylases. Significantly, intactness of the C methylation catalytic sites of ...[more]