Cyclodextrin-PEI-Tat Polymer as a Vector for Plasmid DNA Delivery to Placenta Mesenchymal Stem Cells.

ABSTRACT: This study aims to modify a cyclodextrin-PEI-based polymer, PEI-?-CyD, with the TAT peptide for plasmid DNA delivery to placenta mesenchymal stem cells (PMSCs). By using the disulfide exchange between the SPDP-activated PEI-?-CyD and TAT peptide, the TAT-PEI-?-CyD polymer was fabricated and the success of this was confirmed by the presence of characteristic peaks for PEI (at ? 2.8-3.2 ppm), CyD (at ? 5.2, 3.8-4.0 and 3.4-3.6 ppm) and TAT (at ? 1.6-1.9 and 6.8-7.2 ppm) in the (1)H NMR spectrum of TAT-PEI-?-CyD. The polymer-plasmid-DNA polyplex could condense DNA at an N/P ratio of 7.0-8.0, and form nanoparticles with the size of 150.6±5.6 nm at its optimal N/P ratio (20/1). By examining the transfection efficiency and cytotoxicity of TAT-PEI-?-CyD, conjugation of the TAT peptide onto PEI-?-CyD was demonstrated to improve the transfection efficiency of PEI-?-CyD in PMSCs after 48 and 96 hours of post-transfection incubation. The viability of PEI-?-CyD-treated PMSCs was shown to be over 80% after 5 h of treatment and 24 h of post-treatment incubation. In summary, this study showed that the TAT-PEI-?-CyD polymer as a vector for plasmid DNA delivery to PMSCs and other cells warrants further investigations. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s12668-011-0010-9) contains supplementary material, which is available to authorized users.

SUBMITTER: Lai WF 

PROVIDER: S-EPMC3460531 | biostudies-literature | 2011 Sep

REPOSITORIES: biostudies-literature