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Library-free methylation sequencing with bisulfite padlock probes.

ABSTRACT: Targeted quantification of DNA methylation allows for interrogation of the most informative loci across many samples quickly and cost-effectively. Here we report improved bisulfite padlock probes (BSPPs) with a design algorithm to generate efficient padlock probes, a library-free protocol that dramatically reduces sample-preparation cost and time and is compatible with automation, and an efficient bioinformatics pipeline to accurately obtain both methylation levels and genotypes from sequencing of bisulfite-converted DNA.

SUBMITTER: Diep D

PROVIDER: S-EPMC3461232 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature

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Library-free methylation sequencing with bisulfite padlock probes.

Diep Dinh D Plongthongkum Nongluk N Gore Athurva A Fung Ho-Lim HL Shoemaker Robert R Zhang Kun K

Nature methods 20120205 3

Targeted quantification of DNA methylation allows for interrogation of the most informative loci across many samples quickly and cost-effectively. Here we report improved bisulfite padlock probes (BSPPs) with a design algorithm to generate efficient padlock probes, a library-free protocol that dramatically reduces sample-preparation cost and time and is compatible with automation, and an efficient bioinformatics pipeline to accurately obtain both methylation levels and genotypes from sequencing ...[more]

PMID: 22306810

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