Ontology highlight
ABSTRACT:
SUBMITTER: Puri AW
PROVIDER: S-EPMC3461347 | biostudies-literature | 2012 Sep
REPOSITORIES: biostudies-literature
Puri Aaron W AW Broz Petr P Shen Aimee A Monack Denise M DM Bogyo Matthew M
Nature chemical biology 20120715 9
Here we report AWP28, an activity-based probe that can be used to biochemically monitor caspase-1 activation in response to proinflammatory stimuli. Using AWP28, we show that apoptosis is triggered upon Salmonella enterica var. Typhimurium infection in primary mouse bone marrow macrophages lacking caspase-1. Furthermore, we report that upon Salmonella infection, inflammasome-mediated caspase-1 activity is required to bypass apoptosis in favor of proinflammatory pyroptotic cell death. ...[more]