Dataset Information
Recombinant adiponectin does not lower plasma glucose in animal models of type 2 diabetes.
Ontology highlight
ABSTRACT: Aims/hypothesis
Several studies have shown that adiponectin can lower blood glucose in diabetic mice. The aim of this study was to establish an effective adiponectin production process and to evaluate the anti-diabetic potential of the different adiponectin forms in diabetic mice and sand rats.Methods
Human high molecular weight, mouse low molecular weight and mouse plus human globular adiponectin forms were expressed and purified from mammalian cells or yeast. The purified protein was administered at 10-30 mg/kg i.p. b.i.d. to diabetic db/db mice for 2 weeks. Furthermore, high molecular weight human and globular mouse adiponectin batches were administered at 5-15 mg/kg i.p. b.i.d. to diabetic sand rats for 12 days.Results
Surprisingly, none of our batches had any effect on blood glucose, HbA1c, plasma lipids or body weight in diabetic db/db mice or sand rats. In vitro biological, biochemical and biophysical data suggest that the protein was correctly folded and biologically active.Conclusions/interpretation
Recombinant adiponectin is ineffective at lowering blood glucose in diabetic db/db mice or sand rats.
SUBMITTER: Tullin S
PROVIDER: S-EPMC3462199 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Publications
Recombinant adiponectin does not lower plasma glucose in animal models of type 2 diabetes.
PloS one 20121001 10
<h4>Aims/hypothesis</h4>Several studies have shown that adiponectin can lower blood glucose in diabetic mice. The aim of this study was to establish an effective adiponectin production process and to evaluate the anti-diabetic potential of the different adiponectin forms in diabetic mice and sand rats.<h4>Methods</h4>Human high molecular weight, mouse low molecular weight and mouse plus human globular adiponectin forms were expressed and purified from mammalian cells or yeast. The purified prote ...[more]