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Video-rate molecular imaging in vivo with stimulated Raman scattering.

ABSTRACT: Optical imaging in vivo with molecular specificity is important in biomedicine because of its high spatial resolution and sensitivity compared with magnetic resonance imaging. Stimulated Raman scattering (SRS) microscopy allows highly sensitive optical imaging based on vibrational spectroscopy without adding toxic or perturbative labels. However, SRS imaging in living animals and humans has not been feasible because light cannot be collected through thick tissues, and motion-blur arises from slow imaging based on backscattered light. In this work, we enable in vivo SRS imaging by substantially enhancing the collection of the backscattered signal and increasing the imaging speed by three orders of magnitude to video rate. This approach allows label-free in vivo imaging of water, lipid, and protein in skin and mapping of penetration pathways of topically applied drugs in mice and humans.

SUBMITTER: Saar BG

PROVIDER: S-EPMC3462359 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature

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Video-rate molecular imaging in vivo with stimulated Raman scattering.

Saar Brian G BG Freudiger Christian W CW Reichman Jay J Stanley C Michael CM Holtom Gary R GR Xie X Sunney XS

Science (New York, N.Y.) 20101201 6009

Optical imaging in vivo with molecular specificity is important in biomedicine because of its high spatial resolution and sensitivity compared with magnetic resonance imaging. Stimulated Raman scattering (SRS) microscopy allows highly sensitive optical imaging based on vibrational spectroscopy without adding toxic or perturbative labels. However, SRS imaging in living animals and humans has not been feasible because light cannot be collected through thick tissues, and motion-blur arises from slo ...[more]

PMID: 21127249

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