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Large-scale quantitative glycoproteomics analysis of site-specific glycosylation occupancy.

ABSTRACT: Disease-associated aberrant glycosylation may be protein specific and glycosylation site specific. Quantitative assessment of glycosylation changes at a site-specific molecular level may represent one of the initial steps for systematically revealing the glycosylation abnormalities associated with a disease or biological state. Comparative quantitative profiling of glycoproteome to provide accurate quantification of site-specific glycosylation occupancy has been a challenging task, requiring a concerted approach drawing from a variety of techniques. In this report, we present a quantitative glycoproteomics method that allows global scale identification and comparative quantification of glycosylation site occupancy using mass spectrometry. We further demonstrated this approach by quantitatively characterizing the N-glycoproteome of human pancreas.

SUBMITTER: Pan S

PROVIDER: S-EPMC3463725 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature

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Large-scale quantitative glycoproteomics analysis of site-specific glycosylation occupancy.

Pan Sheng S Tamura Yasuko Y Chen Ru R May Damon D McIntosh Martin W MW Brentnall Teresa A TA

Molecular bioSystems 20120814 11

Disease-associated aberrant glycosylation may be protein specific and glycosylation site specific. Quantitative assessment of glycosylation changes at a site-specific molecular level may represent one of the initial steps for systematically revealing the glycosylation abnormalities associated with a disease or biological state. Comparative quantitative profiling of glycoproteome to provide accurate quantification of site-specific glycosylation occupancy has been a challenging task, requiring a c ...[more]

PMID: 22892896

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