Dataset Information

Structural confirmation of oligosaccharides newly isolated from sugar beet molasses.

ABSTRACT:

Background

Sugar beet molasses is a viscous by-product of the processing of sugar beets into sugar. The molasses is known to contain sucrose and raffinose, a typical trisaccharide, with a well-established structure. Although sugar beet molasses contains various other oligosaccharides as well, the structures of those oligosaccharides have not been examined in detail. The purpose of this study was isolation and structural confirmation of these other oligosaccharides found in sugar beet molasses.

Results

Four oligosaccharides were newly isolated from sugar beet molasses using high-performance liquid chromatography (HPLC) and carbon-Celite column chromatography. Structural confirmation of the saccharides was provided by methylation analysis, matrix-assisted laser desorption/ionaization time of flight mass spectrometry (MALDI-TOF-MS), and nuclear magnetic resonance (NMR) measurements.

Conclusion

The following oligosaccharides were identified in sugar beet molasses: ?-D-galactopyranosyl-(1- > 6)-?-D-fructofuranosyl-(2 <-> 1)-?-D-glucopyranoside (named ?-planteose), ?-D-galactopyranosyl-(1- > 1)-?-D-fructofuranosyl-(2 <-> 1)-?-D-glucopyranoside (named1-planteose), ?-D-glucopyranosyl-(1- > 6)-?-D-glucopyranosyl-(1 <-> 2)-?-D-fructofuranoside (theanderose), and ?-D-glucopyranosyl-(1- > 3)-?-D-glucopyranosyl-(1 <-> 2)-?-D-fructofuranoside (laminaribiofructose). 1-planteose and laminaribiofructose were isolated from natural sources for the first time.

SUBMITTER: Abe T

PROVIDER: S-EPMC3465250 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Structural confirmation of oligosaccharides newly isolated from sugar beet molasses.

Abe Tatsuya T Horiuchi Kenichi K Kikuchi Hiroto H Aritsuka Tsutomu T Takata Yusuke Y Fukushi Eri E Fukushi Yukiharu Y Kawabata Jun J Ueno Keiji K Onodera Shuichi S Shiomi Norio N

Chemistry Central journal 20120827 1

<h4>Background</h4>Sugar beet molasses is a viscous by-product of the processing of sugar beets into sugar. The molasses is known to contain sucrose and raffinose, a typical trisaccharide, with a well-established structure. Although sugar beet molasses contains various other oligosaccharides as well, the structures of those oligosaccharides have not been examined in detail. The purpose of this study was isolation and structural confirmation of these other oligosaccharides found in sugar beet mol ...[more]

PMID: 22925105

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Dataset Information

Structural confirmation of oligosaccharides newly isolated from sugar beet molasses.

Background

Results

Conclusion

Publications

Structural confirmation of oligosaccharides newly isolated from sugar beet molasses.

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