Protein kinase C ? expression in platelets from patients with acute myocardial infarction.

ABSTRACT:

Objective

Platelets play crucial roles in the pathophysiology of thrombosis and myocardial infarction. Protein kinase C ? (PKC?) is virtually absent in human platelets and its expression is precisely regulated during human megakaryocytic differentiation. On the basis of what is known on the role of platelet PKC? in other species, we hypothesized that platelets from myocardial infarction patients might ectopically express PKC? with a pathophysiological role in the disease.

Methods and results

We therefore studied platelet PKC? expression from 24 patients with myocardial infarction, 24 patients with stable coronary artery disease and 24 healthy subjects. Indeed, platelets from myocardial infarction patients expressed PKC? with a significant frequency as compared to both stable coronary artery disease and healthy subjects. PKC? returned negative during patient follow-up. The forced expression of PKC? in normal donor platelets significantly increased their response to adenosine diphosphate-induced activation and adhesion to subendothelial collagen.

Conclusions

Our data suggest that platelet generations produced before the acute event retain PKC?-mRNA that is not down-regulated during terminal megakaryocyte differentiation. Results are discussed in the perspective of peri-infarctual megakaryocytopoiesis as a critical component of myocardial infarction pathophysiology.

SUBMITTER: Carubbi C 

PROVIDER: S-EPMC3465320 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature