Unknown

Dataset Information

0

Ligand-induced changes in T box antiterminator RNA stability.


ABSTRACT: The T box antiterminator RNA element is an important component of the T box riboswitch that controls the transcription of vital genes in many Gram-positive bacteria. A series of 1,4-disubstituted 1,2,3-triazoles was screened in a fluorescence-monitored thermal denaturation assay to identify ligands that altered the stability of antiterminator model RNA. Several ligands were identified that significantly increased or decreased the melting temperature (T(m) ) of the RNA. The results indicate that this series of triazole ligands can alter the stability of antiterminator model RNA in a structure-dependent manner.

SUBMITTER: Zhou S 

PROVIDER: S-EPMC3466076 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature

altmetric image

Publications

Ligand-induced changes in T box antiterminator RNA stability.

Zhou Shu S   Acquaah-Harrison George G   Jack Karen D KD   Bergmeier Stephen C SC   Hines Jennifer V JV  

Chemical biology & drug design 20111222 2


The T box antiterminator RNA element is an important component of the T box riboswitch that controls the transcription of vital genes in many Gram-positive bacteria. A series of 1,4-disubstituted 1,2,3-triazoles was screened in a fluorescence-monitored thermal denaturation assay to identify ligands that altered the stability of antiterminator model RNA. Several ligands were identified that significantly increased or decreased the melting temperature (T(m) ) of the RNA. The results indicate that  ...[more]

Similar Datasets

| S-EPMC4761420 | biostudies-literature
| S-EPMC2526249 | biostudies-literature
| S-EPMC2789444 | biostudies-literature
| S-EPMC3466059 | biostudies-literature
| S-EPMC8934654 | biostudies-literature
| S-EPMC3741684 | biostudies-literature
| S-EPMC1090546 | biostudies-literature
| S-EPMC1302794 | biostudies-literature
| S-EPMC2656570 | biostudies-literature
| S-EPMC4503719 | biostudies-literature