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Towards quantitative metagenomics of wild viruses and other ultra-low concentration DNA samples: a rigorous assessment and optimization of the linker amplification method.

ABSTRACT: Metagenomics generates and tests hypotheses about dynamics and mechanistic drivers in wild populations, yet commonly suffers from insufficient (

SUBMITTER: Duhaime MB

PROVIDER: S-EPMC3466414 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature

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Towards quantitative metagenomics of wild viruses and other ultra-low concentration DNA samples: a rigorous assessment and optimization of the linker amplification method.

Duhaime Melissa B MB Deng Li L Poulos Bonnie T BT Sullivan Matthew B MB

Environmental microbiology 20120620 9

Metagenomics generates and tests hypotheses about dynamics and mechanistic drivers in wild populations, yet commonly suffers from insufficient (< 1 ng) starting genomic material for sequencing. Current solutions for amplifying sufficient DNA for metagenomics analyses include linear amplification for deep sequencing (LADS), which requires more DNA than is normally available, linker-amplified shotgun libraries (LASLs), which is prohibitively low throughput, and whole-genome amplification, which is ...[more]

PMID: 22713159

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