Dataset Information

Evidence for estrogen receptor expression during medullary bone formation and resorption in estrogen-treated male Japanese quails (Coturnix coturnix japonica).

ABSTRACT: The temporal expression of estrogen receptor (ER)-? and ER-? mRNA was examined in male Japanese quails. Femurs of quails receiving 17?-estradiol underwent RT-PCR and histochemical analysis 1 to 15 days after treatment. Untreated quails were used as controls (day 0). Between days 0 and 5, cells lining the bone endosteal surface differentiated into osteoblasts, which in turn formed medullary bone. Expression of ER-? was already observed on day 0 and increased slightly during bone formation whereas ER-? was hardly detected throughout this process. After osteoclasts appeared on the medullary bone surface, this type of bone disappeared from the bone marrow cavity (days 7˜15). ER-? expression simultaneously decreased slightly and ER-? levels remained very low. These results suggest that estrogen activity mediated by ER-? not only affects medullary bone formation but also bone resorption.

SUBMITTER: Hiyama S

PROVIDER: S-EPMC3467396 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature

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Evidence for estrogen receptor expression during medullary bone formation and resorption in estrogen-treated male Japanese quails (Coturnix coturnix japonica).

Hiyama Shinji S Sugiyama Toshie T Kusuhara Seiji S Uchida Takashi T

Journal of veterinary science 20120901 3

The temporal expression of estrogen receptor (ER)-α and ER-β mRNA was examined in male Japanese quails. Femurs of quails receiving 17β-estradiol underwent RT-PCR and histochemical analysis 1 to 15 days after treatment. Untreated quails were used as controls (day 0). Between days 0 and 5, cells lining the bone endosteal surface differentiated into osteoblasts, which in turn formed medullary bone. Expression of ER-α was already observed on day 0 and increased slightly during bone formation whereas ...[more]

PMID: 23000578

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Project description:BackgroundIn recent decades, efforts to produce more efficient poultry products have increased due to its high demand. Meanwhile, some stressors have a negative impact on poultry efficiency and reproduction. Cadmium (Cd) is a toxic heavy metal with a high potential for inducing reactive oxygen species. On the other hand, coenzyme Q10 (CoQ10), with antioxidant properties, exerts a free radical-neutralizing effect on biological systems under stressful conditions.ObjectivesThis study aimed to determine the effect of dietary CoQ10 supplementation on reproductive variables of Cd-challenged male quails.MethodsTwo hundred and sixteen 42-day-old Japanese quails with a male-to-female ratio of 1:3 were randomly divided into three experimental groups (n = 72) and fed by experimental diets from 9 to 13 weeks of age (woa). Treatments included a negative control (NC): feeding basal diet; positive control (PC): feeding basal diet and Cd administration (1 mg per 100 g body weight at 10 and 11 woa); and CdQ10: dietary supplementation of CoQ10 (900 mg per kg diet) and Cd administration. At 10 and 13 woa, liver and testis, cloacal gland index, sera concentration of malondialdehyde (MDA) and testosterone, total antioxidant capacity (TAC), alanine aminotransferase (ALT), testicular histology, mRNA abundance of Hsp70 and fatty acid profile of testis, as well as hatchability and fertility, were measured.ResultsLiver and testis weights, cloacal gland index, serum concentration of testosterone, ALT, MDA, TAC, mRNA abundance of HSP70, hatchability, and fertility were not affected by the treatments. However, Cd administration decreased seminiferous tubule diameter and seminiferous epithelium thickness (SET) in the PC group compared to the NC group (p < 0.05). The proportion of saturated fatty acids (SFA) in testis tissue was increased, and the proportion of PUFA and n-3 to n-6 PUFA ratio was decreased in the PC group compared to the NC group (p < 0.05). In addition, CoQ10 supplementation ameliorated the effect of Cd on decreasing SFA and increasing n-3 to n-6 PUFA ratio proportions.ConclusionsIn conclusion, Cd exerts several adverse effects on reproductive-associated variables; some, but not all, of them are mitigated by CoQ10 supplementation.

Dataset Information

Evidence for estrogen receptor expression during medullary bone formation and resorption in estrogen-treated male Japanese quails (Coturnix coturnix japonica).

Publications

Evidence for estrogen receptor expression during medullary bone formation and resorption in estrogen-treated male Japanese quails (Coturnix coturnix japonica).

Similar Datasets

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