Ontology highlight
ABSTRACT:
SUBMITTER: Nickels JD
PROVIDER: S-EPMC3471459 | biostudies-literature | 2012 Oct
REPOSITORIES: biostudies-literature
Nickels Jonathan D JD O'Neill Hugh H Hong Liang L Tyagi Madhusudan M Ehlers Georg G Weiss Kevin L KL Zhang Qiu Q Yi Zheng Z Mamontov Eugene E Smith Jeremy C JC Sokolov Alexei P AP
Biophysical journal 20121002 7
We present a detailed analysis of the picosecond-to-nanosecond motions of green fluorescent protein (GFP) and its hydration water using neutron scattering spectroscopy and hydrogen/deuterium contrast. The analysis reveals that hydration water suppresses protein motions at lower temperatures (<~ 200 K), and facilitates protein dynamics at high temperatures. Experimental data demonstrate that the hydration water is harmonic at temperatures <~ 180-190 K and is not affected by the proteins' methyl g ...[more]