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Variable region identical immunoglobulins differing in isotype express different paratopes.

ABSTRACT: The finding that the antibody (Ab) constant (C) region can influence fine specificity suggests that isotype switching contributes to the generation of Ab diversity and idiotype restriction. Despite the centrality of this observation for diverse immunological effects such as vaccine responses, isotype-restricted antibody responses, and the origin of primary and secondary responses, the molecular mechanism(s) responsible for this phenomenon are not understood. In this study, we have taken a novel approach to the problem by probing the paratope with (15)N label peptide mimetics followed by NMR spectroscopy and fluorescence emission spectroscopy. Specifically, we have explored the hypothesis that the C region imposes conformational constraints on the variable (V) region to affect paratope structure in a V region identical IgG(1), IgG(2a), IgG(2b), and IgG(3) mAbs. The results reveal isotype-related differences in fluorescence emission spectroscopy and temperature-related differences in binding and cleavage of a peptide mimetic. We conclude that the C region can modify the V region structure to alter the Ab paratope, thus providing an explanation for how isotype can affect Ab specificity.

SUBMITTER: Janda A

PROVIDER: S-EPMC3471687 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature

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Variable region identical immunoglobulins differing in isotype express different paratopes.

Janda Alena A Eryilmaz Ertan E Nakouzi Antonio A Cowburn David D Casadevall Arturo A

The Journal of biological chemistry 20120828 42

The finding that the antibody (Ab) constant (C) region can influence fine specificity suggests that isotype switching contributes to the generation of Ab diversity and idiotype restriction. Despite the centrality of this observation for diverse immunological effects such as vaccine responses, isotype-restricted antibody responses, and the origin of primary and secondary responses, the molecular mechanism(s) responsible for this phenomenon are not understood. In this study, we have taken a novel ...[more]

PMID: 22930758

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Variable region identical immunoglobulins differing in isotype express different paratopes.

Publications

Variable region identical immunoglobulins differing in isotype express different paratopes.

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