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MicroRNA miR-214 regulates ovarian cancer cell stemness by targeting p53/Nanog.

ABSTRACT: Previous studies have shown aberrant expression of miR-214 in human malignancy. Elevated miR-214 is associated with chemoresistance and metastasis. In this study, we identified miR-214 regulation of ovarian cancer stem cell (OCSC) properties by targeting p53/Nanog axis. Enforcing expression of miR-214 increases, whereas knockdown of miR-214 decreases, OCSC population and self-renewal as well as the Nanog level preferentially in wild-type p53 cell lines. Furthermore, we found that p53 is directly repressed by miR-214 and that miR-214 regulates Nanog through p53. Expression of p53 abrogated miR-214-induced OCSC properties. These data suggest the critical role of miR-214 in OCSC via regulation of the p53-Nanog axis and miR-214 as a therapeutic target for ovarian cancer.

SUBMITTER: Xu CX

PROVIDER: S-EPMC3471722 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature

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MicroRNA miR-214 regulates ovarian cancer cell stemness by targeting p53/Nanog.

Xu Cheng-Xiong CX Xu Meng M Tan Lei L Yang Hua H Permuth-Wey Jennifer J Kruk Patricia A PA Wenham Robert M RM Nicosia Santo V SV Lancaster Johnathan M JM Sellers Thomas A TA Cheng Jin Q JQ

The Journal of biological chemistry 20120827 42

Previous studies have shown aberrant expression of miR-214 in human malignancy. Elevated miR-214 is associated with chemoresistance and metastasis. In this study, we identified miR-214 regulation of ovarian cancer stem cell (OCSC) properties by targeting p53/Nanog axis. Enforcing expression of miR-214 increases, whereas knockdown of miR-214 decreases, OCSC population and self-renewal as well as the Nanog level preferentially in wild-type p53 cell lines. Furthermore, we found that p53 is directly ...[more]

PMID: 22927443

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