Microbial diversity of bovine mastitic milk as described by pyrosequencing of metagenomic 16s rDNA.

ABSTRACT: Dairy cow mastitis is an important disease in the dairy industry. Different microbial species have been identified as causative agents in mastitis, and are traditionally diagnosed by bacterial culture. The objective of this study was to use metagenomic pyrosequencing of bacterial 16S rRNA genes to investigate bacterial DNA diversity in milk samples of mastitic and healthy dairy cows and compare the results with those obtained by classical bacterial culture. One hundred and thirty-six milk samples were collected from cows showing signs of mastitis and used for microbiological culture. Additionally, 20 milk samples were collected from healthy quarters. Bacterial DNA was isolated from the same milk samples and the 16S rRNA genes were individually amplified and pyrosequenced. Discriminant anal

SUBMITTER: Oikonomou G 

PROVIDER: S-EPMC3474744 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature