Ontology highlight
ABSTRACT:
SUBMITTER: Shi J
PROVIDER: S-EPMC3475181 | biostudies-literature | 2007 May
REPOSITORIES: biostudies-literature
Shi Jinjun J Yang Tinglu T Kataoka Sho S Zhang Yanjie Y Diaz Arnaldo J AJ Cremer Paul S PS
Journal of the American Chemical Society 20070413 18
The present studies explore multivalent ligand-receptor interactions between pentameric cholera toxin B subunits (CTB) and the corresponding membrane ligand, ganglioside GM1. CTB binding was monitored on supported phospholipid bilayers coated on the walls and floors of microfluidic channels. Measurements were made by total internal reflection fluorescence microscopy (TIRFM). Apparent dissociation constants were extracted by fitting the binding data to both the Hill-Waud and Langmuir adsorption i ...[more]