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ABSTRACT:
SUBMITTER: Chung JK
PROVIDER: S-EPMC3475495 | biostudies-literature | 2012 Sep
REPOSITORIES: biostudies-literature
Chung Jean K JK Thielges Megan C MC Lynch Stephen R SR Fayer Michael D MD
The journal of physical chemistry. B 20120829 36
The changes in fast dynamics of HP35 with a double CN vibrational dynamics label (HP35-P(2)) as a function of the extent of denaturation by urea were investigated with two-dimensional infrared (2D IR) vibrational echo spectroscopy. Cyanophenylalanine (PheCN) replaces the native phenylalanine at two residues in the hydrophobic core of HP35, providing vibrational probes. NMR data show that HP35-P(2) maintains the native folded structure similar to wild type and that both PheCN residues share essen ...[more]