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Genomic profiling of a human leukemic monocytic cell-line (THP-1) exposed to alpha particle radiation.


ABSTRACT: This study examined alpha (?-) particle radiation effects on global changes in gene expression in human leukemic monocytic cells (THP-1) for the purposes of mining for candidate biomarkers that could be used for the development of a biological assessment tool. THP-1 cells were exposed to ?-particle radiation at a dose range of 0 to 1.5 Gy. Twenty-four hours and three days after exposure gene expression was monitored using microarray technology. A total of 16 genes were dose responsive and classified as early onset due to their expression 24 h after exposure. Forty-eight transcripts were dose responsive and classified as late-onset as they were expressed 72 h after exposure. Among these genes, 6 genes were time and dose responsive and validated further using alternate technology. These transcripts were upregulated and associated with biological processes related to immune function, organelle stability and cell signalling/communication. This panel of genes merits further validation to determine if they are strong candidate biomarkers indicative of ?-particle exposure.

SUBMITTER: Chauhan V 

PROVIDER: S-EPMC3477555 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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Genomic profiling of a human leukemic monocytic cell-line (THP-1) exposed to alpha particle radiation.

Chauhan Vinita V   Howland Matthew M  

TheScientificWorldJournal 20121011


This study examined alpha (α-) particle radiation effects on global changes in gene expression in human leukemic monocytic cells (THP-1) for the purposes of mining for candidate biomarkers that could be used for the development of a biological assessment tool. THP-1 cells were exposed to α-particle radiation at a dose range of 0 to 1.5 Gy. Twenty-four hours and three days after exposure gene expression was monitored using microarray technology. A total of 16 genes were dose responsive and classi  ...[more]

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