Ontology highlight
ABSTRACT:
SUBMITTER: Shi Y
PROVIDER: S-EPMC3478657 | biostudies-literature | 2012 Oct
REPOSITORIES: biostudies-literature
Shi Yonghong Y Dierckx Anke A Wanrooij Paulina H PH Wanrooij Sjoerd S Larsson Nils-Göran NG Wilhelmsson L Marcus LM Falkenberg Maria M Gustafsson Claes M CM
Proceedings of the National Academy of Sciences of the United States of America 20120924 41
Transcription factor A (TFAM) functions as a DNA packaging factor in mammalian mitochondria. TFAM also binds sequence-specifically to sites immediately upstream of mitochondrial promoters, but there are conflicting data regarding its role as a core component of the mitochondrial transcription machinery. We here demonstrate that TFAM is required for transcription in mitochondrial extracts as well as in a reconstituted in vitro transcription system. The absolute requirement of TFAM can be relaxed ...[more]