Ontology highlight
ABSTRACT:
SUBMITTER: Egbert RG
PROVIDER: S-EPMC3479488 | biostudies-literature | 2012 Oct
REPOSITORIES: biostudies-literature
Egbert Robert G RG Klavins Eric E
Proceedings of the National Academy of Sciences of the United States of America 20120827 42
The parameters in a complex synthetic gene network must be extensively tuned before the network functions as designed. Here, we introduce a simple and general approach to rapidly tune gene networks in Escherichia coli using hypermutable simple sequence repeats embedded in the spacer region of the ribosome binding site. By varying repeat length, we generated expression libraries that incrementally and predictably sample gene expression levels over a 1,000-fold range. We demonstrate the utility of ...[more]