Mapping the nerve architecture of diabetic human corneas.

ABSTRACT:

Objective

To investigate the entire human corneal nerve architecture of donors with different durations of insulin-dependent diabetes mellitus (IDDM).

Design

Experimental study.

Participants and controls

Sixteen fresh human eyes from 8 diabetic donors (aged 43-66 years, with IDDM for 2-17 years) and 12 eyes from 6 normal donors (aged from 44-67 years) were obtained from the National Disease Research Interchange (NDRI).

Methods

After fixation, corneas were stained with mouse monoclonal anti-β-Tubulin III antibody, and images were acquired to build a whole view of the corneal nerve architecture. The same corneas were used for both whole-mount and cross-section examination.

Main outcome measures

Corneal epithelial nerve density was calculated on the basis o

SUBMITTER: He J 

PROVIDER: S-EPMC3480080 | biostudies-literature | 2012 May

REPOSITORIES: biostudies-literature