Ontology highlight
ABSTRACT:
SUBMITTER: Guha S
PROVIDER: S-EPMC3480190 | biostudies-literature | 2012 Nov
REPOSITORIES: biostudies-literature
Guha Sudipto S Perry Sarah L SL Pawate Ashtamurthy S AS Kenis Paul J A PJ
Sensors and actuators. B, Chemical 20121101
This paper reports a method for fabricating multilayer microfluidic protein crystallization platforms using different materials to achieve X-ray transparency and compatibility with crystallization reagents. To validate this approach, three soluble proteins, lysozyme, thaumatin, and ribonuclease A were crystallized on-chip, followed by on-chip diffraction data collection. We also report a chip with an array of wells for screening different conditions that consume a minimal amount of protein solut ...[more]