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Label-acquired magnetorotation for biosensing: An asynchronous rotation assay.

ABSTRACT: This paper presents a novel application of magnetic particles for biosensing, called label-acquired magnetorotation (LAM). This method is based on a combination of the traditional sandwich assay format with the asynchronous magnetic bead rotation (AMBR) method. In label-acquired magnetorotation, an analyte facilitates the binding of a magnetic label bead to a nonmagnetic solid phase sphere, forming a sandwich complex. The sandwich complex is then placed in a rotating magnetic field, where the rotational frequency of the sandwich complex is a function of the amount of analyte attached to the surface of the sphere. Here, we use streptavidin-coated beads and biotin-coated particles as analyte mimics, to be replaced by proteins and other biological targets in future work. We show this sensing method to have a dynamic range of two orders of magnitude.

SUBMITTER: Hecht A

PROVIDER: S-EPMC3480196 | biostudies-literature | 2011 Feb

REPOSITORIES: biostudies-literature

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Label-acquired magnetorotation for biosensing: An asynchronous rotation assay.

Hecht Ariel A Kinnunen Paivo P McNaughton Brandon B Kopelman Raoul R

Journal of magnetism and magnetic materials 20110201 3-4

This paper presents a novel application of magnetic particles for biosensing, called label-acquired magnetorotation (LAM). This method is based on a combination of the traditional sandwich assay format with the asynchronous magnetic bead rotation (AMBR) method. In label-acquired magnetorotation, an analyte facilitates the binding of a magnetic label bead to a nonmagnetic solid phase sphere, forming a sandwich complex. The sandwich complex is then placed in a rotating magnetic field, where the ro ...[more]

PMID: 23105167

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