Ontology highlight
ABSTRACT:
SUBMITTER: Meier JL
PROVIDER: S-EPMC3483022 | biostudies-literature | 2012 Oct
REPOSITORIES: biostudies-literature
Meier Jordan L JL Yu Abigail S AS Korf Ian I Segal David J DJ Dervan Peter B PB
Journal of the American Chemical Society 20121010 42
Genomic applications of DNA-binding molecules require an unbiased knowledge of their high affinity sites. We report the high-throughput analysis of pyrrole-imidazole polyamide DNA-binding specificity in a 10(12)-member DNA sequence library using affinity purification coupled with massively parallel sequencing. We find that even within this broad context, the canonical pairing rules are remarkably predictive of polyamide DNA-binding specificity. However, this approach also allows identification o ...[more]