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Aptamer-nanoparticle assembly for logic-based detection.

ABSTRACT: In this work, gold nanoparticles perform Boolean logic operations in response to two proangiogenic targets important in cancer diagnosis and treatment: PDGF and VEGF. In the absence of protein target, gold nanoparticles are initially dispersed as a red solution; the addition of target proteins causes nanoparticle aggregation, turning the solution blue, as well as the release of dye-labeled aptamer probes, which causes an increase in fluorescence. These outputs constitute an AND or OR gate for simultaneous protein detection. We believe this logic-gate-based detection system will become the basis for novel rapid, cheap, and reliable sensors for diagnostic applications.

SUBMITTER: Shukoor MI

PROVIDER: S-EPMC3483412 | biostudies-literature | 2012 Jun

REPOSITORIES: biostudies-literature

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Aptamer-nanoparticle assembly for logic-based detection.

Shukoor Mohammed I MI Altman Meghan O MO Han Da D Bayrac Abdullah Tahir AT Ocsoy Ismail I Zhu Zhi Z Tan Weihong W

ACS applied materials & interfaces 20120618 6

In this work, gold nanoparticles perform Boolean logic operations in response to two proangiogenic targets important in cancer diagnosis and treatment: PDGF and VEGF. In the absence of protein target, gold nanoparticles are initially dispersed as a red solution; the addition of target proteins causes nanoparticle aggregation, turning the solution blue, as well as the release of dye-labeled aptamer probes, which causes an increase in fluorescence. These outputs constitute an AND or OR gate for si ...[more]

PMID: 22650355

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