Heterologous production of the lantibiotic Ala(0)actagardine in Escherichia coli.
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ABSTRACT:
SUBMITTER: Shi Y
PROVIDER: S-EPMC3485686 | biostudies-literature | 2012 Nov
REPOSITORIES: biostudies-literature
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Heterologous production of the lantibiotic Ala(0)actagardine in Escherichia coli.
Shi Yanxiang Y Bueno Alejandro A van der Donk Wilfred A WA
Chemical communications (Cambridge, England) 20121101 89
We report the heterologous production of Ala(0)actagardine in E. coli by co-expression of the substrate peptide GarA and its modification enzymes GarM and GarO. The activity of GarO, a luciferase-like monooxygenase that introduces the unique sulfoxide group of actagardine, was also investigated in vitro. ...[more]
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