Ontology highlight
ABSTRACT:
SUBMITTER: Ichinose H
PROVIDER: S-EPMC3485957 | biostudies-literature | 2012 Nov
REPOSITORIES: biostudies-literature
Applied and environmental microbiology 20120831 22
We cloned two glycoside hydrolase family 74 genes, the sav_1856 gene and the sav_2574 gene, from Streptomyces avermitilis NBRC14893 and characterized the resultant recombinant proteins. The sav_1856 gene product (SaGH74A) consisted of a catalytic domain and a family 2 carbohydrate-binding module at the C terminus, while the sav_2574 gene product (SaGH74B) consisted of only a catalytic domain. SaGH74A and SaGH74B were expressed successfully and had molecular masses of 92 and 78 kDa, respectively. ...[more]