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Genome update of Botrytis cinerea strains B05.10 and T4.

ABSTRACT: We report here an update of the Botrytis cinerea strains B05.10 and T4 genomes, as well as an automated preliminary gene structure annotation. High-coverage de novo assemblies and reference-based alignments led to a correction of wrong base calls, elimination of sequence gaps, and improved joining of contigs. The new assemblies have substantially lower numbers of scaffolds and a concomitant increase in the N(50).The list of protein-coding genes was generated using the evidence-driven gene predictor Augustus, with expressed sequence tag evidence and RNA-Seq data as input.

SUBMITTER: Staats M

PROVIDER: S-EPMC3486023 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature

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Genome update of Botrytis cinerea strains B05.10 and T4.

Staats Martijn M van Kan Jan A L JA

Eukaryotic cell 20121101 11

We report here an update of the Botrytis cinerea strains B05.10 and T4 genomes, as well as an automated preliminary gene structure annotation. High-coverage de novo assemblies and reference-based alignments led to a correction of wrong base calls, elimination of sequence gaps, and improved joining of contigs. The new assemblies have substantially lower numbers of scaffolds and a concomitant increase in the N(50).The list of protein-coding genes was generated using the evidence-driven gene predic ...[more]

PMID: 23104368

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