Ontology highlight
ABSTRACT:
SUBMITTER: Williams JP
PROVIDER: S-EPMC3487385 | biostudies-literature | 2008 Dec
REPOSITORIES: biostudies-literature
Williams Jon P JP Wu Jianqiang J Johansson Gunnar G Rizvi Tilat A TA Miller Shyra C SC Geiger Hartmut H Malik Punam P Li Wenling W Mukouyama Yoh-suke YS Cancelas Jose A JA Ratner Nancy N
Cell stem cell 20081201 6
Defining growth factor requirements for progenitors facilitates their characterization and amplification. We characterize a peripheral nervous system embryonic dorsal root ganglion progenitor population using in vitro clonal sphere-formation assays. Cells differentiate into glial cells, smooth muscle/fibroblast (SM/Fb)-like cells, and neurons. Genetic and pharmacologic tools revealed that sphere formation requires signaling from the EGFR tyrosine kinase. Nf1 loss of function amplifies this proge ...[more]