Mass spectrometric analyses of phosphatidylcholines in alkali-exposed corneal tissue.

ABSTRACT:

Purpose

The aims were to determine whether exposure to sodium hydroxide results in predictable changes in phosphatidylcholine (PC) in corneal tissue and if PC profile changes correlate to exposure duration. PCs are major components of the cell membrane lipid bilayer and are often involved in biological processes such as signaling.

Methods

Enucleated porcine (n = 140) and cadaver human eyes (n = 20) were exposed to water (control) and 11 M NaOH. The corneas were excised and lipids were extracted using the Bligh and Dyer method with suitable modifications. Class-specific lipid identification was carried out using a ratiometric lipid standard on a TSQ Quantum Access Max mass spectrometer. Protein amounts were determined using Bradford assays.

Results

Control and alkali-t

SUBMITTER: Crane AM 

PROVIDER: S-EPMC3487488 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature