Experimental phasing using zinc anomalous scattering.

ABSTRACT: Zinc is a suitable metal for anomalous dispersion phasing methods in protein crystallography. Structure determination using zinc anomalous scattering has been almost exclusively limited to proteins with intrinsically bound zinc(s). Here, it is reported that multiple zinc ions can easily be charged onto the surface of proteins with no intrinsic zinc-binding site by using zinc-containing solutions. Zn derivatization of protein surfaces appears to be a largely unnoticed but promising method of protein structure determination.

SUBMITTER: Cha SS 

PROVIDER: S-EPMC3489106 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature