Ontology highlight
ABSTRACT:
SUBMITTER: Strijbis K
PROVIDER: S-EPMC3490390 | biostudies-literature | 2012 Jun
REPOSITORIES: biostudies-literature
Strijbis Karin K Spooner Eric E Ploegh Hidde L HL
Traffic (Copenhagen, Denmark) 20120323 6
Sortagging is a versatile method for site-specific modification of proteins as applied to a variety of in vitro reactions. Here, we explore possibilities of adapting the sortase method for use in living cells. For intracellular sortagging, we employ the Ca²⁺-independent sortase A transpeptidase (SrtA) from Streptococcus pyogenes. Substrate proteins were equipped with the C-terminal sortase-recognition motif (LPXTG); we used proteins with an N-terminal (oligo)glycine as nucleophiles. We show that ...[more]