Improved classification of breast cancer peptide and protein profiles by combining two serum workup procedures.

ABSTRACT:

Purpose

Detection of breast cancer at early stage increases patient's survival. Mass spectrometry-based protein analysis of serum samples is a promising approach to obtain biomarker profiles for early detection. A combination of commonly applied solid-phase extraction procedures for clean-up may increase the number of detectable peptides and proteins. In this study, we have evaluated whether the classification performance of breast cancer profiles improves by using two serum workup procedures.

Methods

Serum samples from 105 breast cancer patients and 202 healthy volunteers were processed according to a standardized protocol implemented on a high-end liquid-handling robot. Peptide and protein enrichments were carried out using weak-cation exchange (WCX) and reversed-phase (RP

SUBMITTER: Velstra B 

PROVIDER: S-EPMC3491194 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature