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Fluorescence behavioral imaging (FBI) tracks identity in heterogeneous groups of Drosophila.

ABSTRACT: Distinguishing subpopulations in group behavioral experiments can reveal the impact of differences in genetic, pharmacological and life-histories on social interactions and decision-making. Here we describe Fluorescence Behavioral Imaging (FBI), a toolkit that uses transgenic fluorescence to discriminate subpopulations, imaging hardware that simultaneously records behavior and fluorescence expression, and open-source software for automated, high-accuracy determination of genetic identity. Using FBI, we measure courtship partner choice in genetically mixed groups of Drosophila.

SUBMITTER: Ramdya P

PROVIDER: S-EPMC3492344 | biostudies-literature | 2012

REPOSITORIES: biostudies-literature

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Fluorescence behavioral imaging (FBI) tracks identity in heterogeneous groups of Drosophila.

Ramdya Pavan P Schaffter Thomas T Floreano Dario D Benton Richard R

PloS one 20121107 11

Distinguishing subpopulations in group behavioral experiments can reveal the impact of differences in genetic, pharmacological and life-histories on social interactions and decision-making. Here we describe Fluorescence Behavioral Imaging (FBI), a toolkit that uses transgenic fluorescence to discriminate subpopulations, imaging hardware that simultaneously records behavior and fluorescence expression, and open-source software for automated, high-accuracy determination of genetic identity. Using ...[more]

PMID: 23144871

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