Ontology highlight
ABSTRACT:
SUBMITTER: Zajac P
PROVIDER: S-EPMC3492873 | biostudies-literature | 2012
REPOSITORIES: biostudies-literature
Zajac Pawel P Ahmadian Afshin A
Scientific reports 20121108
In this work we present a targeted gene expression strategy employing trinucleotide threading (TnT) amplification and massive parallel sequencing. We have previously shown that TnT combined with array readout accurately monitors expression levels. However, with this detection strategy spurious products go undetected. Accordingly, we adapted the TnT protocol to massive parallel sequencing to acquire an unbiased view of the entire TnT-generated product population. In this manner we investigated th ...[more]