Dataset Information

Mapping the differential distribution of proteoglycan core proteins in the adult human retina, choroid, and sclera.

ABSTRACT:

Purpose

To examine the presence and distribution of proteoglycan (PG) core proteins in the adult human retina, choroid, and sclera.

Methods

Postmortem human eye tissue was dissected into Bruch's membrane/choroid complex, isolated Bruch's membrane, or neurosensory retina. PGs were extracted and partially purified by anion exchange chromatography. Trypsinized peptides were analyzed by tandem mass spectrometry and PG core proteins identified by database search. The distribution of PGs was examined by immunofluorescence microscopy on human macular tissue sections.

Results

The basement membrane PGs perlecan, agrin, and collagen-XVIII were identified in the human retina, and were present in the internal limiting membrane, blood vessel walls, and Bruch's membrane. The hyalectans versican and aggrecan were also detected. Versican was identified in Bruch's membrane, while aggrecan was distributed throughout the retina, choroid, and sclera. The cartilage link protein HAPLN1 was abundant in the interphotoreceptor matrix and sclera, while HAPLN4 (brain link protein 2) was found throughout the retina and choroid. The small leucine-rich repeat PG (SLRP) family members biglycan, decorin, fibromodulin, lumican, mimecan, opticin, and prolargin were present, with different patterns of distribution in the retina, choroid, and sclera.

Conclusions

A combination of proteomics and immunohistochemistry approaches has provided for the first time a comprehensive analysis of the presence and distribution of PG core proteins throughout the human retina, choroid, and sclera. This complements our knowledge of glycosaminoglycan chain distribution in the human eye, and has important implications for understanding the structure and functional regulation of the eye in health and disease.

SUBMITTER: Keenan TD

PROVIDER: S-EPMC3493184 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature

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Publications

Mapping the differential distribution of proteoglycan core proteins in the adult human retina, choroid, and sclera.

Keenan Tiarnan D L TD Clark Simon J SJ Unwin Richard D RD Ridge Liam A LA Day Anthony J AJ Bishop Paul N PN

Investigative ophthalmology & visual science 20121107 12

<h4>Purpose</h4>To examine the presence and distribution of proteoglycan (PG) core proteins in the adult human retina, choroid, and sclera.<h4>Methods</h4>Postmortem human eye tissue was dissected into Bruch's membrane/choroid complex, isolated Bruch's membrane, or neurosensory retina. PGs were extracted and partially purified by anion exchange chromatography. Trypsinized peptides were analyzed by tandem mass spectrometry and PG core proteins identified by database search. The distribution of PG ...[more]

PMID: 23074202

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Dataset Information

Mapping the differential distribution of proteoglycan core proteins in the adult human retina, choroid, and sclera.

Purpose

Methods

Results

Conclusions

Publications

Mapping the differential distribution of proteoglycan core proteins in the adult human retina, choroid, and sclera.

Similar Datasets

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